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Image Search Results
Journal: International Journal of Oncology
Article Title: miR-382 inhibits breast cancer progression and metastasis by affecting the M2 polarization of tumor-associated macrophages by targeting PGC-1α
doi: 10.3892/ijo.2022.5416
Figure Lengend Snippet: miR-382 inhibits the metastasis of 4T1 breast cancer cells by inhibiting the polarization of M2 macrophages in vivo . (A and B) Mouse macrophages transfected with miR-382-overexpressing lentivirus were mixed with 4T1 cells at a 4:1 ratio and subcutaneously inoculated into BALB/c mice, and breast tumor growth was monitored. The long and short diameters of the tumors were measured every 5 days, and the tumor volume was calculated using the formula ab 2 /2. the maximum diameter of tumors did not exceed 2 cm. (C and D) After observation, the mice were sacrificed, lung tissues were harvested for hematoxylin and eosin staining, the tumor growth rate was determined, and the number of metastases was calculated. Images were captured under 400× magnification. (E and F) At the end of the experiment, the mice were sacrificed, tumor tissues were harvested and embedded in paraffin, and immunohistochemical staining was performed. Images were captured under ×400 magnification. Data are presented as the mean ± SD of three independent experiments ( ** P<0.01).
Article Snippet: Tumors derived from 4T1 cells and lung tissues were removed and fixed in 4% paraformaldehyde (BL539A, Biosharp) for 24 h. Following tissue dewaxing,
Techniques: In Vivo, Transfection, Staining, Immunohistochemical staining